Document Abstract
Fluoride is a natural component of the earth’s crust and soil. Small amounts of fluorides are present in water, air,
plants, and animals, which when enters the food chain causes toxic effects to the ecosystem due to its
bioaccumulation. The fluoride ions act as enzymatic poisons, inhibiting enzyme activity and ultimately interrupting
metabolic process such as glycolysis and synthesis of proteins. After oral uptake, water-soluble fluorides are rapidly
and almost completely absorbed in the gastrointestinal tract. As soon as fluoride is absorbed, blood fluoride levels
increase. The study deals with the acute exposure of sodium fluoride to Clarias batrachus under laboratory
condition. Biological samples were collected after every 24 hours for estimation of biochemical parameters (Total
protein, ACP, ALP, GOT, GPT). The percent fluoride accumulation and the bio-concentration factor were studied
after 96 hr of exposure. The result indicates significant changes in the biochemical parameters. The statistical
analysis was done using five-way ANOVA.